Composite

Part:BBa_I13516:Design

Designed by: jkm   Group: iGEM04_MIT   (2005-06-06)


Screening Plasmid Intermediate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1791
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Deletion of the C at position 1194. This is right at the end of I0500, after the transcriptional start of the downstream gene but before the RBS. Shouldn't make a difference, but needs to be checked out.


Source

References